rabbit polyclonal anti rpl7 nb100 2269 antibodies (Novus Biologicals)
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rabbit polyclonal anti rpl7 nb100 2269 antibodies
Rabbit Polyclonal Anti Rpl7 Nb100 2269 Antibodies, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 92/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rabbit+polyclonal+anti+rpl7/RPL7+Antibody/pmc09135405-427-24-30
Average 92 stars, based on 5 article reviews
Rabbit Polyclonal Anti Rpl7 Nb100 2269 Antibodies, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 92/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rabbit+polyclonal+anti+rpl7/RPL7+Antibody/pmc09135405-427-24-30
Average 92 stars, based on 5 article reviews
rabbit polyclonal anti rpl7 nb100 2269 antibodies - by Bioz Stars,
2026-09
92/100 stars
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1) Product Images from "CLUH controls astrin-1 expression to couple mitochondrial metabolism to cell cycle progression"
Article Title: CLUH controls astrin-1 expression to couple mitochondrial metabolism to cell cycle progression
Journal: eLife
doi: 10.7554/eLife.74552
Figure Legend Snippet: ( A, B ) Western blots of reciprocal co-IPs of endogenous CLUH, astrin, and kinastrin in HeLa cells. Two different antibodies have been used to pull down CLUH (1, 2). Asterisk marks IgG light chain. ( C ) Scheme of human SPAG5 cDNA with indicated UTRs and ORFs. Closeup shows positions of the uATG and ATG1–6 with surrounding Kozak sequences. ( D, E ) Western blots of HeLa cells overexpressing FLAG-tagged astrin constructs. Pan-actin was used as loading control. ( F ) Western blots of reciprocal co-IPs of endogenous CLUH and overexpressed FLAG-tagged astrin full length (ATG1-SPAG5) or a N-terminal deleted variant (Δ151-SPAG5) in WT and CLUH KO HeLa cells. Pan-actin was used as loading control for input samples. Asterisks indicate additional astrin bands appearing upon overexpression of the N-terminal deleted variant. ( G ) Confocal immunofluorescence pictures of HeLa cells overexpressing FLAG-tagged astrin-1 and astrin-2 (SPAG5), astrin-1 (ATG3 + 4 + 5 GGG -SPAG5) or astrin-2 (ATG1 GGG -SPAG5) alone stained for FLAG (green) and CLUH (red). DAPI was used to stain nuclei (blue). Small boxes in left corners show a ×4 magnified area of boxed regions. Scale bar, 10 µm. ( H ) Polysome profiling of HeLa cells chemically crosslinked with dithiobis succinimidyl propionate (DSP). At the top, absorbance profile at 254 nm of the fractions is shown with indicated peaks of 40S and 60S ribosomal subunits, 80S monosome and polysomes; at the bottom the corresponding western blots of the fractions are shown. RPL7 was used as a marker for ribosomes. Figure 1—source data 1. Uncropped blots for . Figure 1—source data 2. Uncropped blots for . Figure 1—source data 3. Unedited blots for .
Techniques Used: Western Blot, Construct, Control, Variant Assay, Over Expression, Immunofluorescence, Staining, Marker
Figure Legend Snippet:
Techniques Used: CRISPR, Expressing, Derivative Assay, Selection, Recombinant, Plasmid Preparation, Construct, Control, Transfection, Sequencing, cDNA Synthesis
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Purification:Article Title: Cell-type-specific isolation of ribosome-associated mRNA from complex tissues Article Snippet: .. We used the following antibodies: Mouse monoclonal anti-HA (purified and ascites, 1:1,000 dilution; Covance); Incubation:Article Title: Cholinergic neuron gene expression differences captured by translational profiling in a mouse model of Alzheimer's disease. Article Snippet: Cholinergic neurotransmission is impaired in Alzheimer’s disease (AD), and loss of basal forebrain cholinergic neurons is a key component of disease pathogenicity and symptomatology.. To explore the molecular basis of this cholinergic dysfunction, we paired translating ribosome affinity purification (TRAP) with RNA sequencing (TRAP-Seq) to identify the actively translating mRNAs in anterior forebrain cholinergic neurons in the TgCRND8 mouse model of AD.. Bioinformatic analyses revealed the downregulation of 67 of 71 known cholinergic-related transcripts, consistent with cholinergic neuron dysfunction in TgCRND8 mice, as well as transcripts related to oxidative phosphorylation, neurotrophins, and ribosomal processing. Blocking Assay:Article Title: Cholinergic neuron gene expression differences captured by translational profiling in a mouse model of Alzheimer's disease. Article Snippet: Cholinergic neurotransmission is impaired in Alzheimer’s disease (AD), and loss of basal forebrain cholinergic neurons is a key component of disease pathogenicity and symptomatology.. To explore the molecular basis of this cholinergic dysfunction, we paired translating ribosome affinity purification (TRAP) with RNA sequencing (TRAP-Seq) to identify the actively translating mRNAs in anterior forebrain cholinergic neurons in the TgCRND8 mouse model of AD.. Bioinformatic analyses revealed the downregulation of 67 of 71 known cholinergic-related transcripts, consistent with cholinergic neuron dysfunction in TgCRND8 mice, as well as transcripts related to oxidative phosphorylation, neurotrophins, and ribosomal processing. |
